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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Protein Expr Purif. 2007 Dec 3;58(2):263–268. doi: 10.1016/j.pep.2007.11.009

Figure 1.

Figure 1

SDS-PAGE showing the expression of the His6-SUMO-brazzein fusion in various host strains. Lane 1: Molecular weight markers (Invitrogen). Lanes 2/3: −IPTG / +IPTG (BL21 (DE3)/RI Codon Plus). Lanes 4/5: −IPTG / +IPTG (BL21 (DE3)/RIL Codon Plus). Lanes 6/7: −IPTG / +IPTG (BL21 (DE3)/RILP Codon Plus). Lanes 8/9: −IPTG / +IPTG (BL21(DE3)/pLysS). Lanes 10/11: −IPTG / +IPTG (Rosetta (DE3)). The His6-SUMO-brazzein fusion is observed at 23.5 kD and brazzein at 6.4 KD.