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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Protein Expr Purif. 2007 Dec 3;58(2):263–268. doi: 10.1016/j.pep.2007.11.009

Figure 2.

Figure 2

Medium-scale expression and purification of His6-SUMO-brazzein produced from BL21 (DE3)/ PRIL Codon Plus cells. Lane 1: Molecular weight markers (Invitrogen). Lane 2: −IPTG. Lane 3: total cell lysates. Lane 4: supernatant. Lane 5: pellet. Lanes 6–9: Ni-NTA column fractions (2 to 5). Lane 10: purified fusion protein. Lane 11: SUMO 1 protease cleavage product after 30 min. Lane 12: cleavage product after 60 min.