Table 2.

Phenotypic revertants were identified in the M2 generation among the progeny of EMS-treated cpr1 plants.

Source			Number of M2 plants phenotype
			cpr1	Intermediate	Wild-type
Treatment	Phenotype in M1	Family
Experiment #1
Mock3A	cpr1	c1	99	0	0
Mock	cpr1	c2	98	0	0
30 mM EMS	cpr1	c3	66	9	0
30 mM EMS3B	cpr1	c4	51	3	0
30 mM EMS3C	Chimeric	c5*	42	0	43
30 mM EMS	Chimeric	c6	21	0	18
Experiment #2
Treatment
Mock	cpr1	c7	85	0	0
Mock	cpr1	c8	96	0	0
30 mM EMS	cpr1	c9	50	3	0
30 mM EMS	cpr1	c10	28	0	0
30 mM EMS	Chimeric	c11	4	0	31
30 mM EMS	Chimeric	c12	31	0	74

Plants with slightly curled leaves that were wider than cpr1 were called intermediate in phenotypic categorization. Chimeric M1 plants were those that developed chimeric sectors of wild type-looking leaves and stems. ^3A, ^3B, and ^3C: Lines for which representative M2 plants are shown in Figure 3A to 3C.

*: P < 0.05 by chi square test with expected ratio of 5:3 or 1:3 and degree of freedom = 1.