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Detection and quantification of synthetic let-7b in the presence of a complex background using the miR-Q assay and mirVana™ qRT-PCR Detection Kit within the same qPCR run according to the mirVana protocol. The purchasable let-7b assay showed a sensitivity limit of 20 fM synthetic let-7b (black columns), while the sensitivity limit of the miR-Q assay turned out to be 2 fM synthetic let-7b (white columns). Each column represents the mean (± SD) of three measurements.
