Table 1.

Values of binding time estimated from the binding curves β(t_I) as well as the association rate constants calculated according (11), (12) and the association rate constant for free streptavidin, take from [13].

			Association rate constant
Samples	MNP-concentration cP (nmol/1)	binding time tb (s)	eq. (11) ka $({10}^3\frac{\text{l}}{\text{mol s}})$	eq. (12) ka $({10}^3\frac{\text{l}}{\text{mol s}})$	Ref. [13] ka $({10}^3\frac{\text{l}}{\text{mol s}})$
without agitation
MNP*SAV	2	9400 ± 1000	54 ± 9	270 ± 45	5000
MNP*AB	23	3600 ± 350	12 ± 2	60 ± 10
with agitation
MNP*SAV	2	4400 ± 220	116 ± 20	580 ± 100	5000
MNP*SAV	6	2700 ± 60	63 ± 2	315 ± 10	5000
MNP*AB	23	807 ± 100	55 ± 10	275 ± 50