Table 1.
Results of PARP inhibitor synthetic lethal siRNA screen
| siRNA SMARTpool® | KU0058948-sensitivity Z-score | Growth % siCONTROL |
|---|---|---|
| IMPK | −8.33 | 96 |
| PNKP | −8.21 | 54 |
| ATR | −7.27 | 106 |
| TTBK1 | −6.31 | 60 |
| STK36 | −5.50 | 65 |
| PLK1 | −5.15 | 6 |
| STK35 | −5.09 | 77 |
| CDC2 | −4.17 | 55 |
| ADRA1A | −4.15 | 56 |
| PIP5K2B | −4.10 | 48 |
| PLK3 | −3.99 | 73 |
| ITGB1BP1 | −3.94 | 86 |
| STK22C | −3.85 | 87 |
| AKAP1 | −3.64 | 48 |
| FLJ34389 | −3.35 | 63 |
| GSK3A | −3.32 | 78 |
| CHEK1 | −3.26 | 42 |
| CDK5 | −3.18 | 106 |
| ATM | −3.12 | 92 |
| MAPK12 | −3.11 | 59 |
| PMVK | −3.07 | 83 |
| DAPK1 | −3.07 | 66 |
| MGC5601 | −3.05 | 52 |
| PRKD2 | −3.00 | 97 |
| Results of siRNA screen displayed in Z-score order with siRNA SMARTpool target gene, KU0058948 PARP-inhibitor sensitivity Z-score and the effect of siRNA on cell growth in vehicle-alone plates. The first 24 siRNAs are statistically significant, with a Z-score of less than −3. Hits in bold italic have well-documented roles in the DNA-damage response, and have previously been shown to sensitise to PARP inhibitors. Hits in boldface are revalidated in subsequent experiments as being on-target. Statistically significant hits 21–24 were not further revalidated. |