Figure 4.

C-terminal tyrosines of Syk are required for pre-B-cell responses. (A) Syk^−/− pre-B cells were transduced with the constructs indicated on the right. The data are represented in the same way as in Figure 3, namely forward scatter versus GFP (left panel), pre-BCR (μ surface expression (panel 2), surface expression of κ light chain (κLC) 3 days after IL-7 withdrawal (panel 3) and Ca²⁺ flux after pre-BCR activation with anti-IgM antibodies (panel 4). Data are representative of at least three independent experiments. (B) Quantitation of pre-B-cell differentiation as in Figure 3B. Error bars indicate s.e.m.