Figure 6.

N-glycan at the GB2 VFT interface prevents dimerization with GB1. (A) The schemes depict the experimental approach used to monitor receptor dimers at the cell surface using time-resolved FRET. The FRET signal is measured between an anti-HA antibody linked to a donor molecule (D) and an anti-Flag antibody linked to an acceptor molecule (A). (B) FRET signal between HA-tagged GB1_ASA and Flag-tagged GB2 subunits in cells coexpressing the indicated constructs. (C, D) Amount of HA- and Flag-tagged subunits expressed at the cell surface, respectively, as measured by ELISA. Data are means±s.e. of triplicates from a typical experiment.