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. 2008 May 14;3(5):e2174. doi: 10.1371/journal.pone.0002174

Figure 3. 6AP and GA interaction with ribosomal components is RNA-dependent.

Figure 3

Crude yeast cell extracts were incubated with 6AP beads (left) or GA beads (right). Lane 1: control beads without 6AP or GA, lane 2: chromatography using 6AP or GA beads, lane 3: cellular extracts were treated by RNase A at 100 µg/ml for 20 minutes at room temperature before affinity chromatography experiments. The bound proteins were analyzed by SDS-PAGE followed by silver staining. Note that after treatment of cellular extracts by RNase A, ribosomal constituents are unable to bind to 6AP or GA beads, indicating that interaction between both compounds and the ribosomal constituents is RNA-dependent.