Figure 3. The derived 370A allele of EDAR results in enhanced activation of NF-κB in vitro.

Transfection of a HEK293 cell line with ancestral (370V) and derived (370A) versions of EDAR activate NF-κB to a different extent on both a normal genetic background (comparison on the left) and on a background containing a known disease mutation (375H; comparison on the right). Luciferase activity is driven by EDAR-activated NF-κB. Data was averaged from two independent experiments with reads from at least 9 wells total for each clone. Error bars are standard error of the mean (* p<0.05, ** p<0.01).