Figure 5. Destabilization of primate TRIM5α proteins is correlated with restriction of HIV-1 and SIVmac239.

(A) 293T cells expressing HA-tagged TRIM5α_rh, TRIM5α_tam (tamarin monkey), TRIM5α_sp (spider monkey), and were pretreated for 1 hour with cycloheximide then exposed to stocks of VSV-G pseudotyped HIV-GFP, SIV-GFP, or media alone (Mock). (B). Quantitation of relative TRIM5α levels. Data shown are from one representative of three independent experiments.