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. 2008 May 23;4(5):e1000074. doi: 10.1371/journal.ppat.1000074

Figure 7. HIV-1 specifically induces destabilization of TRIM5α in rhesus macaque cells.

Figure 7

(A) Duplicate cultures of 293T-TRIM5αrh and FRHK-4 cells were transfected on two consecutive days with siRNAs specific for TRIM5αrh (TRIM5α) or a non-targeting control siRNA (NTC). 72 hours after the second transfection, proteins were extracted and analyzed by immunoblotting with a TRIM5α–specific monoclonal antibody. (B) Quantitation of TRIM5αrh levels in siRNA-transfected cells by Odyssey imaging software. TRIM5αrh levels following siRNA knockdown are expressed as the average of the duplicate determinations with the error bars depicting the range of values. (C) FRhK-4 cells were challenged with HIV-GFP(VSV) or media alone (Mock). Zero hour timepoint represents TRIM5α levels in uninfected cells. (D) Quantitation of TRIM5αrh levels. Results shown are from one representative of three independent experiments.