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. 2008 May 23;4(5):e1000074. doi: 10.1371/journal.ppat.1000074

Figure 8. HIV-1-induced turnover of TRIM5α in primate cells specific to restriction and is blocked by the proteasome inhibitor MG132.

Figure 8

(A) Duplicate cultures of FRhK-4 cells were exposed to stocks of VSV-G-pseudotyped HIV-GFP (HIV), SIV-GFP (SIV), or media alone (Mock) for a period of 4 hours then harvested and TRIM5αrh levels quantified by immunoblotting. The zero hour sample corresponds to uninfected cells. (B) Quantitation of TRIM5αrh levels in (A) utilizing Odyssey imaging software. (C) Duplicate cultures of FRhK-4 cells were treated for 1 hour with DMSO or MG132, then exposed for 4 hours to: VSV-G-pseudotyped HIV-GFP with either DMSO or MG132, or media alone containing DMSO or MG132 (Mock). (D) Quantitation of TRIM5αrh levels utilizing Odyssey imaging software. Data in this figure are expressed as the averages of the two determinations, with the error bars spanning the range of values.