Skip to main content
. Author manuscript; available in PMC: 2009 May 16.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Mar 6;369(4):1071–1075. doi: 10.1016/j.bbrc.2008.02.125

Figure 2.

Figure 2

Stabilization of GLA by IFG-preincubation in vitro. GLA was preincubated with IFG at room temperature for 30 min. The EI complex was diluted 50-fold, incubated for indicated length of time at indicated temperature, and then assayed for activity. Concentrations indicated are final concentrations of IFG in EI complex. (A) EI complex was diluted in saline and was heated at 54°C. (B) EI complex was diluted with PBS (pH 7.4 and pH 8.0) and heated at 37°C. (C) EI complex was diluted in human plasma and was heated at 37°C. (D) EI complex was diluted in SDS solutions (0.1% and 0.2%) and incubated at room temperature for 30 min. Enzyme activity is expressed as relative activity to untreated enzyme. For (A–C), results are the mean of duplicate assays and are representative of two independent experiments. For (D), data are presented as mean ± SE (n = 3). *P<0.004, comparison of the enzyme with and without IFG preincubated, two-tailed Student’s t test.