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Elevated phospho-Akt levels in mammary gland of NHERF1 knockout mice. (a) Genotyping of NHERF1 knockout mice. Numbers 1 to 3, 4 to 6, and 7 to 9 represent NHERF1+/+, NHERF1+/-, and NHERF1-/- mice, respectively. (b) Immunodetection of NHERF1 and phospho-Akt (p-Akt; Ser473) expression in mammary gland tissues from mice of respective genotypes. Membrane was stripped and reprobed with β-actin to normalize loading. WT, wild-type.
