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Cells growing as spheroids express stem cell proteins. Immunofluorescence of spheroids formed by A1.8 and RP.1 cells after four passages were stained for Numb, Oct4, and CD133. Cells growing as spheroids were allowed to attach to an eight-well slide, fixed, and stained with indicated antibodies. Staining for Oct4 is visualized in both cell lines, whereas expression of Numb and CD1333 is evident only in RP.1 cells.
