Figure 6.

Adenine nucleotide translocator 2 shRNA inhibited tumor growth in vivo. (a) In vivo experimental schedule and tumor regression induced by adenine nucleotide translocator (ANT) 2 shRNA. Balb/c nude mice were challenged with 5 × 10⁶ MDA-MB-231 cells by subcutaneous injection into the right flanks and then treated with PBS, scramble shRNA or respective ANT2 shRNA-1, shRNA-2 and shRNA-3 vectors supplemented with Lipofectamine 2000 by intratumoral injection on day 21, day 23 and day 25 post challenge. Tumor sizes were measured by a caliper every week and volumes calculated using m₁² × m₂ × 0.5236 (where m₁ represents the short tumor axis and m₂ the long axis) until day 56 post tumor challenge. (b) On day 32, tumor tissues were isolated and subjected to in situ apoptosis staining (Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay). Bright green dots represent apoptotic bodies.