Table 2.

Relative transcript levels* of repeats in ES cell lines

	Minor satellite	Major satellite	X141
WT (OS25)	0.4 ± 0.1	1.8 ± 0.5	1.8 ± 0.3
Eed KO	7.2 ± 6.5†	18.5 ± 17.0†	1.8 ± 0.2
Dnmt1 KO	0.3 ± 0.1	2.1 ± 0.0	2.0 ± 1.3
Dnmt3a/3b DKO	0.1 ± 0.0	5.7 ± 2.4	1.5 ± 0.9
G9a WT	0.1 ± 0.0	0.7 ± 0.1	0.5 ± 0.7
G9a KO	0.4 ± 0.4	1.0 ± 0.2	ND
Suv39 h1/h2 WT	4.9 ± 0.8	2.5 ± 1. 6	1.1 ± 0.3
Suv39 h1/h2 DKO	4.0 ± 1.3	2.2 ± 0.7	2.1 ± 0.5
Dicer WT	0.2 ± 0.0	4.1 ± 0.0	2.7 ± 0.0
Dicer KO	0.9 ± 0.6	17.4 ± 5.5	4.1 ± 1.2
C2C12 dif	100	100	100

*Levels of repeat sequence RNA were normalized to a house keeping gene (Ube) and expressed as a percentage of the levels detected in differentiated mouse myocytes derived from C2C12 (C2C12 dif), samples in which high levels of major and minor satellite transcripts have previously been detected [56]. Controls without reverse transcriptase were analyzed in parallel to dismiss contamination with genomic DNA.

^†Four independent samples showed variable but increased transcript levels: major satellite, 8.2%, 8.7%, 13.4%, and 43.8% of C2C12; minor satellite, 2.6%, 7.1%, 11.8%, and 13.8% of C2C12. ND, none detected.