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. 2008 May 21;3(5):e2223. doi: 10.1371/journal.pone.0002223

Figure 7. PP2A is involved in localization control of Cdc5.

Figure 7

(A) Cells grown in SCD at 30°C (WT (W303-1B), sch9Δ (YYK823), rim15Δ (YYK831), sit4Δ (BY9004), pph21Δpph22Δ (BY9943)) or 24°C (tap42-11, (CY5755)) expressing EGFP-Cdc5 were shifted to the indicated temperature for 1 h, and EGFP-Cdc5 was observed by fluorescent microscope. (B) Overexpression of CDC5 suppresses growth defect of pph21Δpph22Δ. Wild-type, pph21Δpph22Δ, and sit4Δ cells harboring high-copy CDC5 plasmid were grown on YEPD plates at 30 and 37°C for 2 days. Note that growth defect of sit4Δ was exacerbated by CDC5 overexpression.