Figure 3. Moesin is required for estrogen-induced cytoskeletal remodeling.

A) T47-D cells were transiently transfected with antisense (2 µM antisense MOESIN) moesin phosphorotioate oligonucleotides (PONs) for 0, 60 or 72 hours and moesin expression was assayed with western analysis. B and C) T47-D cells were transiently transfected with vehicle or antisense (2 µM antisense MOESIN) or sense (2 µM sense MOESIN) moesin phosphorotioate oligonucleotides (PONs) for 72 hours and then treated for 15 min with 10 nM E2. Moesin expression was checked by staining with a specific Ab linked to FITC (green labeling). Actin fibers were stained with phalloidin-Texas Red (red labeling) and nuclei were counterstained with DAPI (blue labeling). F) shows the analytic results obtained by using Leica QWin image analysis and processing software showing the mean thickness of the cell membrane as well as the intensity of actin and moesin staining in the cytoplasm, membrane or extracellular compartment. The results are derived from the sampling of five areas of the cell membrane of thirty different random cells. The areas of minimum and maximum cell membrane thickness were always included. The results are the mean±SD of the measurements. * = p<0.05 vs. vehicle-transfected control.