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. 2008 May 21;3(5):e2240. doi: 10.1371/journal.pone.0002240

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Pask et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Functional analysis of the thylacine non-coding DNA fragment. (a) Diagram of transgene construct. 4 copies of a 264-bp fragment containing the Thylacine Col2a1 enhancer (TcyCol2a1) region was ligated to the human b-globin minimal promoter (black box) and ligated to lacZpA. (b–e) X-gal stained 14.5 dpc TcyCol2a1-lacZpA transgenic mouse embryo showing varying levels of reporter gene expression within the developing cartilage (blue). (f) Non-transgenic littermate, negative control fetus. (g) Top panel; Magnified image of forelimb from fetus in (b) black line indicates the plane of section shown in (g) bottom panel. Bottom panel; Histological section of transgenic forelimb digit, showing lacZ-expressing chondrogenic tissue (blue) counterstained with eosin (pink).