Figure 2.

Reverse Northern blot. DNA (2 μg) of the amplified gene fragment of each of the purified clones, along with various amount of α-actin DNA (0.5, 1, 2, or 4 μg) were blotted on Hybond-N⁺ nylon transfer membrane using the slot-blot apparatus. After denaturation, neutralization and DNA fixation, the filters containing the spotted DNA were hybridized to ³²P-labelled cDNA probes. (A) Autoradiogram obtained after Reverse Northern blot hybridization of the spotted cloned gene fragments to a probe prepared by reverse transcription of 30 μg of total RNA purified from the pooled tumour biopsies of six cervical cancer patients. (B) Autoradiogram obtained after Reverse Northern blot hybridization of the spotted cloned gene fragments to a probe prepared by reverse transcription of 30 μg of total RNA purified from the corresponding pooled normal tissue biopsies of the same six cervical cancer employed in (A). (C) The identity and orientation of the cloned gene fragments as spotted on the filter membranes.