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. 2002 Jan 21;86(2):168–178. doi: 10.1038/sj.bjc.6600039

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Copyright © 2002 The Cancer Research Campaign

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Intra-individual variability of MFI for ζ-chain expression and the per cent of ζ-chain-negative cells in CD3⁺, CD4⁺ and CD8⁺ T cell subsets obtained from six normal donors (C1–6). The samples were drawn at three different time points 1 month apart. PBMC were cryopreserved and tested in the same assay to establish the biologic variation within each individual evaluated over a 3 month period. The solid line connects the median values. Of note, the percentages of ζ-chain-negative T cells are very low in normal controls, except for CD8⁺ T cells in C5.