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. 2002 Jan 21;86(2):250–256. doi: 10.1038/sj.bjc.6600035

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Copyright © 2002 The Cancer Research Campaign

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Detection of oestrogen receptor mRNAs by RT–PCR. (A) ERβ, (B) ERα, (C) Alpha-actin. In all panels, lane M 100 bp ladders, lanes 1–9 breast tumour samples, the negative control lane (−) contained a sample prepared without reverse transcriptase. Note that although a cDNA specific for ERβ was amplified from all samples, the amount of ERα cDNA amplified from the same sample set was highly variable.