Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2002 Feb 1;86(3):456–462. doi: 10.1038/sj.bjc.6600058

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2002 The Cancer Research Campaign

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Western blot of ZD 1839 treated cell lines using (A) the anti-phosphotyrosine PY20 antibody, (B) the phospho-ERK NEB 9101 antibody and (C) the phospho-AKT^S473 NEB 9271 antibody. Blots are shown for the PE01, PE04, SKOV-3, OVCAR-5 and A2780 cell lines. ZD 1839 was added 30 min prior to TGFα (1 nM) addition and lysates were collected after 15 min exposure to TGFα. Amounts of protein loaded were 75 μg lane⁻¹ for the phosphotyrosine gel and 35 μg for the phospho-ERK and phospho-AKT gels. Exposure times varied from 20 s to 2 min but were the same for each antibody.