Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2002 Feb 12;86(4):636–644. doi: 10.1038/sj.bjc.6600100

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2002 Cancer Research UK

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Functional expression of P2-receptors. (A) Biphasic increase of [Ca²⁺]_i after ATP application (100 μM) in Ca²⁺-containing bath solution. (B) Monophasic [Ca²⁺]_i rise after ATP application (100 μM) in Ca²⁺-free solution. (C) Application of UTP (100 μM) during prolonged ATP stimulation (100 μM) failed to elicit any further response. (D) Application of ATP (100 μM) during prolonged UTP stimulation (100 μM) did not cause any further rise of [Ca²⁺]_i. The [Ca²⁺]_i is given as the fluorescence ratio (ΔF340/F380 nm) of the Ca²⁺-sensitive dye fura-2. Representative single-cell tracings out of three independent experiments with 20 or more cells per incubation are shown. (E) Different nucleotides in equimolar concentration (100 μM) induced distinct increases of [Ca²⁺]_i in KYSE-140 cells measured under [Ca²⁺]_e-containing conditions. Values are given as the peak increase above basal [Ca²⁺]_i of resting cells. The basal level of resting cells amounted to 58±7 nM. Means±s.e.m. of 44 to 98 cells per nucleotide are shown.