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. 2002 Mar 4;86(5):737–743. doi: 10.1038/sj.bjc.6600153

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Copyright © 2002 Cancer Research UK

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RT–PCR amplification (A) and relative (GAPDH-normalised) mRNA levels (B) of survivin and its alternative splice variants in gastric cancer. (A) Two μg of total RNA was reversely transcribed and amplified by polymerase chain reaction. PCR-products were electrophoresed and visualised by ethidium bromide staining. Four arbitrarily selected examples of each UICC disease stage are shown. M, molecular weight marker; NC, negative control (H₂O); survivin (431 bp); survivin-2B (500 bp); survivin-ΔEx3 (329 bp). (B) Survivin is the dominant transcript. P-values were calculated by the Wilcoxon-test.