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. 2002 Apr 22;86(8):1250–1256. doi: 10.1038/sj.bjc.6600221

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Copyright © 2002 Cancer Research UK

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CXCR4 and CXCL12α mRNA expression using real-time RT–PCR. mRNA expression in four RCC cell lines (A-498, CAKI-1, CAKI-2 and HA-7), tumour (T) and adjacent normal non-malignant tissue (N) of 10 patients with RCC is demonstrated as a ratio corrected for that of RPS9 mRNA expression. The median T/N ratio for all patients was 29.6 (range, 2.3–85.2) for CXCR4, and 0.36 (range: 0.1–2.3) for CXCL12α, respectively, displaying a substantial upregulation of the receptor in malignant kidney tissue, while its ligand was less expressed compared to the corresponding normal renal tissue.

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