Figure 5.

Effects of CM101 on isolated CNS from mouse embryos. Control recording was made by using two intracellular microelectrodes placed on each side of the spinal cord lesion on day 1 (A) and day 5 (B) in vitro. Compression and crush of isolated tissue at the level of T10 of the spinal cord produced significant reduction of conduction of electric stimuli on day 1 (C). On day 5 (D), in the absence of CM101, no electric stimuli were detected after multiple and increased stimulation. In spinal cord tissue cultured with CM101 (0.3 μg/ml), a slightly longer latency of transmitted excitation was seen on day 1 (E) and significant improvement in conductivity was observed on day 5 (F). B-1, D-1, and F-1 are expansions of the time axis of day 5 recordings. Action potentials (B-1 and F-1) suggest neuronal components of pulse transmission that are absent in untreated CNS (D-1).