Figure 7.

Effects of CM101 on cultured mouse spinal cord neurons traumatized by axonal mechanical compression and stimulated with 3-s pulses of 10 μM GABA (horizontal lines below readout). CM101 in vitro reversed GABA-mediated depolarizing responses in injured mammalian central neurons in the culture (G and I). The oscilloscope traces A, B, and C represent recordings of hyperpolarization from the control uninjured neurons. The oscilloscope traces D, E, and F represent recordings of depolarization from the mechanically injured neurons on days 1, 2, and 5 after trauma, respectively. The oscilloscope traces G, H, and I represent recordings from the mechanically injured neurons on days 1, 2, and 5 after trauma, treated with CM101-supplemented (0.3 μg/ml) culture medium. Calibration at the right refers to respective row(s).