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. 2002 Jun 17;86(12):1914–1919. doi: 10.1038/sj.bjc.6600331

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Copyright © 2002 Cancer Research UK

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Expression of AMF/PHI/NL mRNA in the transfectants: (A) Northern blot analysis of AMF/PHI/NL mRNA in the HIF-1α-transfectants (PCI-10/H2 and PCI-10/H3) and vector-transfectant (PCI-10/V2) under non-hypoxic conditions. AMF/PHI/NL mRNA expression in PCI-10 cells under non-hypoxic (N) and hypoxic (H) conditions were shown as controls. (B) Northern blot analysis of dominant negative HIF-1α (dnHIF-1α) mRNA in the transfectants. (C) Northern blot analysis of AMF/PHI/NL mRNA expression in the dnHIF-1α -transfectants (PCI-43/dnH3 and PCI-43/dnH7) and the vector-transfectant (PCI-43/vV3) under non-hypoxic (N) and hypoxic (H) conditions. RNAs were extracted from the cells incubated under non-hypoxic (N) and hypoxic (H) conditions for 24 h and then Northern blot analysis was performed.