Figure 6.

The effects of IGFBP-3 and TGF-β on ceramide-induced death of the MCF-10A cells. Graphs (A + B) represent the per cent of dead cells. MCF-10A cells were (A) pre-incubated with IGFBP-3 (100 ng ml⁻¹) for 24 h followed by a co-incubation with an apoptotic dose of C2 for a further 24 h (where ***C2>CT; P<0.001, *IGFBP-3+C2<C2; P<0.05) or (B) treated with TGF-β (5 ng ml⁻¹) for 3 days with or without spiking with an apoptotic dose of C2 on the penultimate day (where **C2>CT; P<0.01, *TGF-β+C2>C2; P<0.05). Graph represents the mean of experiments that were each performed in triplicate at least three times.