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. 2007 Oct 4;585(Pt 1):203–215. doi: 10.1113/jphysiol.2007.141119

Figure 5.

Figure 5

Forkhead transcription factor (FoxO) protein expression in diaphragm skeletal muscle with mechanical ventilation A, representative Western blot and ponceau-stained nitrocellulose membranes to demonstrate even loading and transfer for the analysis of FoxO1 (Ser-256), phosphorylated FoxO3 (Thr-32), and phosphorylated FoxO4 (Ser-193) and nuclear FoxO1, total FoxO3, and total FoxO4 proteins. B, phosphorylated FoxO1 (Ser-256) protein. C, phosphorylated FoxO3 (Thr-32) protein expression. D, phosphorylated FoxO4 (Ser-256) protein expression. E, total nuclear FoxO1 protein expression. F, total nuclear FoxO3 protein expression. G, total nuclear FoxO4 protein expression. Values are normalized to control values and presented as means ± s.e.m.* Significantly (P < 0.05) different from control values.