Table 3.
Basal levels of mitochondrial biogenesis markers and citrate synthase activity in the soleus and EDL of CON and NOS knockout mice
CON | eNOS−/− | nNOS−/− | |
---|---|---|---|
SOLEUS | |||
PGC-1β mRNA (arbitrary units) | 1.24 ± 0.10 | 1.54 ± 0.12 | 1.53 ± 0.17 |
COX III mRNA (arbitrary units) | 1.07 ± 0.10 | 0.92 ± 0.07 | 1.08 ± 0.13 |
Citrate synthase activity (μmol min−1 (g protein)−1) | 778 ± 16 | 924 ± 62† | 1180 ± 41†# |
EDL | |||
PGC-1β mRNA (arbitrary units) | 0.75 ± 0.11 | 0.85 ± 0.15 | 1.17 ± 0.21 |
COX III mRNA (arbitrary units) | 1.18 ± 0.14 | 1.07 ± 0.14 | 1.64 ± 0.29 |
Citrate synthase activity (μmol min−1 (g protein)−1) | 772 ± 14 | 781 ± 16 | 818 ± 23 |
Values are means ± s.e.m.n = 8 for all groups.
P <0.05 versus CON mice;
P <0.05 versus eNOS−/− mice (one-way ANOVA).