Figure 3.

Percentage changes in skin blood flow in response to local pressure application in young adult and old mice without inhibition (untreated, n = 10 in each group), following the inhibition of NOS and COX using N^ω-nitro-l-arginine and indomethacin (LNNA + INDO, n = 5 in young adult mice and n = 8 in old mice) and following the blockade of endothelium-derived hyperpolarizing factor (EDHF) using apamin and charybdotoxin (APA + ChTX, n = 6 in young adult mice and n = 5 in old mice). Error bars represent s.e.m. * P < 0.05 old mice compared to young adult mice. # P < 0.05 treated compared to age-matched untreated mice.