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. 2007 Nov 1;586(Pt 1):265–282. doi: 10.1113/jphysiol.2007.144501

Figure 1. Corticostriatal transmission in striatal fast-spiking GABAergic interneurons.

Figure 1

A, schematic representation of the horizontal corticostriatal brain slice preparation. The stimulating electrode was placed in layer V of the somatosensory cortex (mean distance from the cortical border of corpus callosum 586 ± 10 μm, n = 110) and the recording electrode in the adjacent striatum at a mean distance from the striatal border of corpus callosum of 543 ± 34 μm. B, characteristic membrane properties and spiking pattern of a fast-spiking GABAergic interneuron. Raw traces show individual voltage responses to a series of 500 ms current pulses from −90 pA to +130 pA with 20 pA increasing current steps (left side) and to +30 pA above AP threshold (middle panel, spike frequency = 40 Hz). Note the linear I–V relationship (right panel). C, fast-spiking GABAergic interneuron EPSCs evoked by cortical layer V (Cx (LV)) stimulations. Black line represents the average of 20 consecutive EPSC raw traces (grey lines). A low variability of cortically evoked EPSC amplitudes should be noted. D, latency distribution of 60 EPSCs recorded in a single GABAergic interneuron (well fitted by a Gaussian curve). Note the short latency centred on 1.7 ms. E, amplitude distribution of 60 EPSCs recorded in the same GABAergic interneuron shown in D (distribution well fitted by a Gaussian curve centred around −121 pA). F, cortically evoked EPSCs on fast-spiking GABAergic interneurons were glutamatergic since they were totally inhibited by AP5 (50 μm) and CNQX (20 μm) treatments. This is illustrated by a representative experiment showing averages of 15 traces recorded in control conditions (‘Control’) and after AP5 and CNQX treatment (‘AP5+CNQX’). G, latency distribution of EPSCs was fitted with one Gaussian function centred on 1.67 ± 0.03 ms (n = 784 EPSCs from 28 GABAergic interneurons). H, SD distribution of averaged EPSC latencies recorded in 28 GABAergic interneurons indicates a monosynaptic transmission between cortex and fast-spiking GABAergic interneurons (mean value of latency SD was 0.23 ± 0.02 ms).