Figure 5. Blockade of glutamate and GABA transporters.

A, left panel, train stimulation-induced disinhibition was tested in control (n = 8) and in the presence of the glutamate uptake inhibitor dl-TBOA (50 μm) and d-APV (50 μm) (n = 8). dl-TBOA did not enhance disinhibition, although glutamate spillover was promoted, as shown in the example traces on the right. B, blockade of GAT-1 by 5 μm NO-711 transiently enhanced train stimulation-induced disinhibition. On the left, reduction of eIPSCs is plotted against the recording time (n = 6). Each data point corresponds to the average of 16 responses. See Fig. 2C for statistical significance. In the middle, example eIPSCs from a single recording: average test responses (black) and conditioned responses (grey) in the first 8 min of recording (1) and after 20 min (2). On the right, example PSCs evoked during train stimulation during the first 8 min of recording (1) and after 20 min (2).