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. 2007 Nov 29;586(Pt 3):717–726. doi: 10.1113/jphysiol.2007.142877

Figure 2. The effects of UTP and BAPTA-AM on apical membrane current of amphotericin B-permeablized monolayers maintained in the presence of 20 nM oestradiol-17β for 4 days.

Figure 2

A, monolayers stimulated with apical UTP (10 μm) exhibited a rapid increase in inward current consistent with movement of K+ down its concentration gradient. The tracing is representative of data from 16 monolayers. Treatment with BAPTA-AM abolished the initial inward current response elicited by UTP and reduced the rate at which outward current developed after stimulation with UTP (n = 5). Attempts to block the outward current by pretreatment with 200 μm NPPB resulted in a decrease in inward current and a reduction in outward current following stimulation with UTP (n = 3). B, bar graph showing the peak inward current elicited by UTP in the absence and presence of 50 μm BAPTA-AM. The data represent the mean ±s.e.m. from 16 control and 5 BAPTA-AM-pretreated monolayers (*indicates a significant decrease in peak inward current compared to control monolayers).