During normothermia, when all sited were perfused with Ringer solution only, CVC did not differ between microdialysis sites (P > 0.05 between sites). During perfusion of 5% DMSO in Ringer, these values increased at both sites (P > 0.05 between sites). Perfusion of the latter site with 2 mm 7-NI in 5% DMSO in Ringer did not change CVC values significantly (P > 0.05 7-NI versus 5% DMSO in Ringer). No such trend was present at the untreated site where 5% DMSO perfusion was maintained. During whole-body cold stress, CVC fell at both sites. The CVC responses to cold stress did not differ between sites (P > 0.05 between sites). During whole-body heating, CVC rose at both sites. The CVC responses to whole-body heat stress differed significantly between untreated and 7-NI-treated sites, thus nNOS antagonism attenuated the cutaneous active vasodilator response to whole-body heat stress. (*P < 0.05 within sites; **P < 0.05 between sites).