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Solution application protocols used in the experiments
| Protocol | Step 1 | Step 2 | Step 3 |
|---|---|---|---|
| Control | ECS 5 s | ACh 250 ms | — |
| Antagonist | ECS + Antag 5 s | ACh + Antag 250 ms | — |
| Onset kinetics | ECS 5 s | ECS + Antag 0–5000 ms | ACh + Antag 250 ms |
| Recovery kinetics | ECS + Antag 5 s | ECS 0–5000 ms | ACh 250 ms |
The perfusion solutions contain extracellular solution (ECS) alone or with 300 μm ACh and/or various concentrations of antagonist (Antag).
