Figure 2. The rate of onset of desensitization is slowed by W55 mutations.

Representative inward current responses due to the rapid and sustained application of ACh (1 mm; indicated by the horizontal bar) for wildtype (A) and mutant (α7-W55A, B; α7-W55C, C) α7 nAChRs. The onset of desensitization half-time values are shown in Table 1. Oocytes were incubated in BAPTA-AM (100 μm) for 1–4 h to inhibit Ca²⁺-activated chloride currents.