Figure 5. Regulation of SMIT1 by the ubiquitin ligase Nedd4-2.
Coexpression of wild type Nedd4-2, but not of the catalytically inactive Nedd4-2 (C962S), significantly down-regulated myo-inositol-induced currents. SGK1 counteracted the inhibiting Nedd4-2 effect and activated SMIT1 currents when coexpressed with inactive Nedd4-2 (C962S). Arithmetic means ± s.e.m. of myo-inositol (2 mm)-evoked currents. * indicates statistically significant difference (P < 0.05) to oocytes injected with SMIT1 alone.