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. 2008 Jan 10;586(Pt 7):1823–1832. doi: 10.1113/jphysiol.2007.149450

Figure 3. Kv7.4 channel expression in the raphe nuclei.

Figure 3

A, representative immunohistochemical staining of Kv7.4 channels in the rat dorsal (DRN) and median raphe (MNR) nucleus of the rat brainstem (10× magnification, 40 μm section). B, further caudal level of the DRN (20× magnification, 40 μm section). The data indicate that Kv7.4 channels are expressed throughout the DRN at the rostral–caudal level. A rabbit polyclonal Kv7.4 channel antibody (Kharkovets et al. 2000) diluted 1: 5000 and biotinylated donkey anti-rabbit IgG (1: 800; Jackson ImmunoResearch Laboratories, West grove, PA, USA) were used. Immunoreactivity was detected by means of the avidin–biotin amplification method using diaminobenzidine as chromagen and amplified using the biotinylated tyramine procedure as previously described (Hansen et al. 2006).