Fig. 2.

Multicolour immunofluorescence of human osteoblasts. (a–g) Six-colour immunofluorescence plus DAPI nuclear staining with spectral image acquisition and (j–o) single-colour immunofluorescence plus DAPI nuclear staining with conventional digital image acquisition. For each picture (a–o) antibodies and filter sets were used as described in Fig. 1. No labelling could be detected for endoglin (CD105) and VCAM-1 (CD106). All scale bars = 50 µm.