Fig. 4.

Relations between Bergmann glia, Purkinje neurons and NG2-glia. Confocal microscopic images of mid-sagittal sections of P19 cerebellum from GFAP-EGFP mice, immunolabelled for calbindin (A) or NG2 (B), and deconvolved and rotated using Volocity (version 4.0.1; Improvision, UK). (A) Multiple Bergmann glia serve a single Purkinje cell, and each Bergmann glial cell contains many hundreds of independent microdomains which ensheath synapses. (B) NG2-glia process domains encompasses multiple Bergmann glia and form intricate associations with the processes of Bergmann glia that ensheath synapses (inset). NG2-glia receive inputs via these processes from multiple climbing fibres (Lin et al. 2004). Scale bar = 15 µm.