Table 1.
Primary monoclonal antibodies used for immunohistochemistry
| Antigen | Clone | Dilution | Pre-treatment | Source | Reference |
|---|---|---|---|---|---|
| Collagen I | Col I | 1 : 2000 | Hyal (1.5 IU mL−1) & ChABC (0.25 IU mL−1) | Sigma-Aldrich | none |
| Collagen II | CIICI | 1 : 6 | Hyal (1.5 IU mL−1) & ChABC (0.25 IU mL−1) | DSHB | Holmdahl et al. (1986) |
| Collagen III | FH7A | 1 : 4000 | Hyal (1.5 IU mL−1) & ChABC (0.25 IU mL−1) | Sigma-Aldrich | Olsen et al. (1993) |
| Collagen VI | 5C6 | 1 : 10 | Hyal (1.5 IU mL−1) & ChABC (0.25 IU mL−1) | DSHB | Hessle & Engvall (1984) |
| Keratan sulphate | 5D4 | 1 : 1500 | none | B. Caterson | Caterson et al. (1983) |
| Chondroitin 4 sulphate | 2B6 | 1 : 1500 | ChAC (0.25 IU mL−1) | B. Caterson | Caterson et al. (1985) |
| Chondroitin-4- & Dermatan-sulphate | 2B6 | 1 : 1500 | ChABC (0.25 IU mL−1) | B. Caterson | Caterson et al. (1985) |
| Chondroitin-6-sulphate | 3B3 | 1 : 150 | ChABC (0.25 IU mL−1) | B. Caterson | Caterson et al. (1985) |
| Chondroitin-6-sulphate (oversulphated) | 7D4 | 1 : 350 | none | B. Caterson | Caterson et al. (1990) |
| Cartilage oligomeric matrix protein (COMP) (rat) | HC484D1 | 1 : 20 | none | Serotec | none |
| Link protein | 9/30/8A4 | 1 : 10 | Reduction and alkylation then ChAC (0.25 IU mL−1) | B. Caterson | Calabro et al. (1992) |
| Aggrecan | 12/21/1C6 | 1 : 10 | Reduction and alkylation then ChAC (0.25 IU mL−1) | B. Caterson | Calabro et al. (1992) |
| Versican | 12C5 | 1 : 10 | ChAC (0.25 IU mL−1) | DSHB | Asher et al. (1991, 1995) |
| Tenascin | T2H5 | 1 : 100 | ChAC (0.25 IU mL−1) | Serotec | Verstraeten et al. (1992) |
| Neurofilaments | RT97 | 1 : 10 | none | DSHB | none |
ChAC, chondroitinase AC; ChABC, chondroitinase ABC; Hyal, hyaluronidase; DSHB, Developmental Studies Hybridoma Bank, University of Iowa (USA).