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Intestinal cell cultures obtained from the duodenum, jejunum and ileum reaching confluence (A). Intestinal cell cultures harvested at varying trypsin dissociation times taken from different intestinal regions reaching confluence (B). Intestinal cell cultures taken from different age range of neonates reaching confluence (C). Each point represents the mean ± SE; n = 16 (A) n = 4 (B,C) *P < 0.05, **P < 0.01 and ***P < 0.001 taken as significant differences in the group.
