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Coculture of intestinal preparations at day 1 showing ISMC-like and rounded clusters of neurones (A). By day 2, ISMC developed into relatively uniform layer throughout the culture with intervening areas of characteristically developed myenteric neurones (arrows) (B). By day 5, ISMC were arranged in multilayered regions with intervening neurones showing extending neurites (arrows) from the myenteric neurones in coculture with ISMC. Scale bar = 100 µm.
