Fig. 5.

Cells at day 1 (A) and following differential adhesion to minimize numbers of neurones and fibroblasts resulted in cultures of ISMC-like and few clusters of neurones (arrows) (B). At day 2, the neurotoxicity following pre-treatment with scorpion venom (30 µg mL⁻¹) increased proliferation of ISMC (C). Incubation with second dose of scorpion venom resulted in cultures displaying multilayered spindle-shaped cells, which were uniform throughout the culture (D). Scale bar = 100 µm.