Fig. 9.

Neuronal preparations with dual-label staining directed to 5HT₃ receptors (green) and α-smooth muscle actin (red) antibodies for confirmation of the nature of the purified neurones. DAPI mount was used for labeling cell nucleus (blue). Neuronal cell bodies and extended neurites stained positive to 5HT₃ receptors antibody (A). Negative immunoreactivity to α-smooth muscle actin antibody confirms the absence of the ISMC in the preparation (A). Negative controls showed no staining to both primary antibodies (B). Scale bar = 25 µm.